Nîmes May 2016

Since the discovery of the V617F mutation of tyrosine kinase JAK2 in 2005, other mutations have been described as specific for Myeloproliferative Neoplasms (MPNs) in JAK2 eons 12, 13, 14 (in addition to c1849G>T V617F) and 15, in the exon 10 of MPL, and in the exon 9 of the CALR gene. Moreover, other rare mutations have been described in the JAK2 and MPL genes. Other “non-driving” mutations are also associated with MPNs (CBL, ASXL1, …) and involved in disease progression (EZH2, IDH, TET2). These findings markedly modified understanding of MPN pathophysiology, and modified the diagnostic work-up of MPNs. Different molecular biology techniques dedicated to the detection and/or the quantification of the main MPN mutations have been designed: AS-PCR, ARM PCR, hybridization probe, Taqman probe, HRM, LNA clamped PCR, digital droplet PCR (ddPCR) for absolute quantification and recently, next-generation sequencing (NGS)… MPN&MPNr-EuroNet training school speakers are experts in these technologies, and several are members of scientific organizations such as the European Leukemia Net (ELN), a European network dedicated to the establishment of guidelines, best practices, and validation of techniques.
MPN&MPNr-EuroNet aims to facilitate interactions between experts and early-stage researchers and biologists, to foster synergy, disseminate scientific information and share experience and training in novel techniques. MPN&MPNr-EuroNet training schools meet these objectives, by gathering experts and early-stage researchers and biologists for 3-4 days of informal exchanges on the theoretical and practical aspects of MPN mutation detection and quantification. Since the first MPN&MPNr-EuroNet Training School, organized in Nîmes in May 2011, all MPN&MPNr-EuroNet training schools have pursued these aims and the schools have been warmly appreciated by trainees.

The aims of the Training School are the following:

1. To acquire competences in the various techniques designed to detect the main MPN mutations. The training school is organized in two sessions:
   • Morning plenary session/lectures: Theory of the techniques.
   • Afternoon practical session: applicants will perform data analysis, assisted by experts.
2. To train in NGS data analysis, moving from raw data to documented diagnostic proposal.
3. To get the latest information on the different mutations leading to MPN or to transformation.
4. To develop cooperation between participants and hopefully, generate new, original studies.

The maximum applicant number is 10 so that each trainee can practice in ideal conditions.

Deadline for Registration: April 30th, 2016.
Registration fees : 300,00 € – No scholarship for this year.
Fore more information, contact Dr. Sylvie Hermouet or Dr. Serge Carillo

MPN&MPN-EuroNet offers three scholarships of a maximum of 800 Euros per trainee. To apply for a scholarship, applicant must send a mail to Dr Beatriz Bellosillo-Paricio explaining why she/he wishes to attend the training school and why a scholarship is needed. The applicant should also provide a short CV. Click on the following link to send a mail to Dr Beatriz Bellosillo-Paricio. Please upload here the CV template

The deadline for application to a scholarship is February 15th, 2014.

Applicants will be informed of the decision regarding attribution of scholarship via e-mail by March 1st, 2013.

Molecular diagnostic, myeloproliferative neoplasms, JAK2 mutation, MPL mutation, allelic burden quantification, CALR mutations

Click on the following link to get more info about MPN&MPNr-EuroNet.