Aims
The aims of the MPN&MPNr-EuroNet’s Training School to be held in Coimbra, Portugal, October 20-22, 2011 are the following:
- To get the latest information on the different mutations leading to congenital erythrocytosis.
- To acquire competences in the various techniques used for the molecular diagnosis of congenital erythrocytosis. The training school is organized in two sessions (see programme):
- Morning plenary session/lectures: theory.
- Afternoon practical session: applicants will perform the different assays, assisted by experts, and analyze the results.
- To start a cooperative group which could share samples, reference materials and hopefully, generate original studies.
Objectives
Applicants will acquire theoretical and practical competences enabling them to set up in their own laboratory the assays necessary for the diagnosis of congenital erythrocytosis.
Keywords
Molecular diagnostic, congenital erythrocytosis
More info
Click on the following link to get more info about MPN&MPNr-EuroNet.
Abstract Submission
The maximum applicant number is 10 so that each trainee can practice in ideal conditions.
Applicants will benefit from the molecular biology platform of the University Hospital of Nîmes. They will practice under the guidance of experts. In order to maximize and facilitate exchanges, experts will be present with trainees for the whole day.
Deadline for application: Monday April 11th, 2011.
Registration fees : 250,00 €.
Who is concerned ?
All biologists interested in learning the techniques used for the diagnosis of congenital erythrocytosis. The training school will deal with:
- High oxygen affinity Hbs and DPGM deficiency – Biochemical and molecular genetics
- Congenital methaemoglobinemias
- EPOR, HIF2A, PHD2 mutations
- VHL gene – Chuvash polycythemia and other mutations
- Jak2 exon 12 mutations: HRM, SSCP, Sequenciation
- rs12343867 analysis of the JAK2 GGCC haplotype: HRM and Sequenciation
Trainees will benefit from the molecular biology platform of the Pediatric Hospital of Coimbra. They will practice under the guidance of experts. In order to maximize and facilitate exchanges, experts will be present with trainees for the whole day.
The maximum applicant number is 12 so that each trainee can practice in ideal conditions.
Deadline for application: September 30th, 2011.
Registration fees : 200,00 €.
Preliminary Programme of the training school
Molecular Diagnosis of Congenital Erythrocytosis
WEDNESDAY OCTOBER 19th, 2011 |
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| 19:00 | Welcome Reception, Hotel Tryp, Coimbra, Portugal | |
THURSDAY OCTOBER 20th, 2011 |
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| 09:00-09:30 | Welcome to the COST Training School | Leticia Ribeiro, Coimbra, Portugal |
| 09:30-10:30 | Classification & Genetic Basis of Congenital Erythrocytosis | Mary Frances McMullin, Belfast, UK |
| 10:30-11:30 | Clinical presentation of Congenital Erythrocytosis | Francesca Pierdomenico, Lisboa, Portugal |
| Coffee break | ||
| 12:00-13:00 | How to investigate a Congenital Erythrocytosis | Holger Cario, Ulm, Germany |
| Lunch at the Pediatric Hospital cafeteria | ||
| 14:00-14:30 | High oxygen affinity Hbs – Biochemical & molecular genetics | Celeste Bento, Coimbra, Portugal |
| 14:30-15:30 | Lab work High affinity Hb mutations detection : P50, HPLC, IEF |
Umbelina Rebelo, Luis Relvas, Coimbra, Portugal |
| 15:30-17:30 | Lab work HBB, HBA genes study |
Celeste Bento, Ana Catarina Oliveira, Coimbra, Portugal |
| 20:00 | Dinner | |
FRIDAY OCTOBER 21th, 2011 |
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| 09:00-10:00 | Hypoxia and erythrocytosis | Mary Frances McMullin, Belfast, UK |
| 10:00-11:00 | Germ line mutations in genes involved in the HIF pathway : tumor suppressor genes associated with erythrocytosis? | Betty Gardie, Nantes, France |
| Coffee break | ||
| 11:30-13:00 | Discussion of clinical cases | Leticia Ribeiro, Tabita Maia, Coimbra, Portugal |
| Lunch at the Pediatric Hospital cafeteria | ||
| 14:00-15:00 | Molecular genetic approaches to the study of erythrocytosis genes | Janet Pereira, Coimbra, Portugal |
| 15:00-18:00 | Lab work EPOR, HIF2A, PHD2 and VHL mutation detection |
Helena Almeida, Catarina Silva Pinto, Coimbra, Portugal |
| Dinner | ||
| Interactive session with the speakers of the day | ||
SATURDAY OCTOBER 22th, 2011 |
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| 09:00-10:00 | Familial Polycythemia Vera | Holger Cario, Ulm, Germany |
| 10:00-11:30 | Jak2 mutations (V617F, exon 12 and GGCC haplotype): Detection by ASO-PCR, HRM, SSCP and Sequenciation | Margarida Coucelo, Michele Horta, Coimbra, Portugal |
| Coffee break | ||
| 12:00-13:00 | Interactive session: Discussion of results | |
| Lunch possible, on request, at the Pediatric Hospital cafeteria | ||
Place of Meeting
Molecular Hematology Unit
Pediatric Hospital Coimbra
Av. Afonso Romão
Coimbra – Portugal
Tel : 00351 96 4455227
To visualize the Pediatric Hospital location
click here.
Contact with the organizers
Before to contact the organizers, please read carefully the information provided on the web site. Questions may ONLY be asked through the contact webpage.
How to register, submit a contribution, pay registration fees, get invoice ?
For registration, submission of contribution … select Registration on the menu bar.
Pediatric Hospital Coimbra
Hotels and accommodation
Each participant has to make his/her own room reservation.
See below the nearest recommended hotels (15 min by bus, 30 min walking)
Please mention your participation to the MPN Training School when booking to benefit from our special rates.
- Residencial Alentejana, Rua Dr. António Henrique Seco Nber 1, 3000-145 Coimbra.
The hotel “Residencial Alentejana” is familiar, mainly with double and triple rooms. The price for the students in the course is 23 €/night in a shared room or 45 € in a single room. - Hotel Tryp Coimbra, Av Armando Gonçalves Lote 20, 3000 059 Coimbra.
The Tryp Hotel is more comfortable and the rate is 60 €/night/ room. - Coimbra Youth Hostel Rua Dr. Henriques Seco 14, 3000-145 Coimbra Portugal, GPS 40° 12′ 40,09″ N – 8° 24′ 51,11″ W
How to get there ?
- by plane (low cost):
- by train:
- From Porto or Lisboa to Coimbra-B;
Choose Alfa & Intercidades
- From Porto or Lisboa to Coimbra-B;
- by car:
- See viamichelin
or use your GPS: Av. Afonso Romão, Coimbra
- See viamichelin
Moving down town …
The hotels are located near the center, the University, and, almost … the river, so it’s easy and nice to go there walking.
Bus Line 7 leads from each of the above mentioned hotels to the Pedriatric Hospital.
The bus station is at about 50 m from each hotel. Time schedule : every 10 to 15 min.
The journey takes 10 to 15 min to reach the hospital.
The price of each ticket is 1 €.
Coimbra
- City map
- Coimbra City Touristic information
