Aims
The aims of MPN&MPNr-EuroNet’s Training School to be held in Nîmes, France, May 11-13 2011 are the following:
- to acquire competences in the various techniques designed to detect the JAK2 and MPL mutations characteristic of Philadelphia-negative myeloproliferative neoplasms (MPN). The training school is organized in two sessions:
- Morning plenary session/lectures: theory of the techniques.
- Afternoon practical session: applicants will perform the different assays, assisted by experts.
- to get the latest information on the different mutations leading to MPN or to MPN transformation (GGCC/46.1 haplotype, TET2, CBP Alpha, CBL, ASXL-1, IDH-1, EZH, LNK…)
- to start a cooperative group which could share samples, reference materials and hopefully, generate original studies.
Objectives
Applicants will acquire theoretical and practical competences enabling them to set up in their own laboratory the assays necessary for the detection and quantification of JAK2V617F, as well as assays for the detection of others mutations in the JAK2 and MPL genes.
The training school will deal with:
- allelic burden quantification of the JAK2V617F mutation, using real time or LNA clamped PCR
- HRM (High Resolution Melting analysis) detection of “second line mutations” of JAK2 and MPL: JAK2 exon12 mutations; mutations in other JAK2 exons; MPL exon10 mutations
- Information and contacts with laboratories expert in the detection of rare mutations (TET2, CBP Alpha, CBL, ASXL-1, IDH-1, EZH, LNK …).
Keywords
Molecular diagnostic, myeloproliferative neoplasms, JAK2 mutation, MPL mutation, allelic burden quantification
More info
Click on the following link to get more info about MPN&MPNr-EuroNet.
Abstract Submission
The maximum applicant number is 10 so that each trainee can practice in ideal conditions.
Applicants will benefit from the molecular biology platform of the University Hospital of Nîmes. They will practice under the guidance of experts. In order to maximize and facilitate exchanges, experts will be present with trainees for the whole day.
Deadline for application: Monday April 11th, 2011.
Registration fees : 250,00 €.
Scholarships
MPN&MPN-EuroNet offers five scholarships. To apply for a scholarship, applicant must send a mail to Mrs.Elodie Hervio explaining why she/he wishes to attend the training school and why a scholarship is needed. The applicant should also provide a short CV. Click on the following link to send a mail to Mrs. Elodie Hervio.
The deadline for application to a scholarship is March 30th, 2011.
Applicants will be informed of the decision regarding attribution of scholarship via e-mail by April 5th, 2011.
Who is concerned ?
The maximum applicant number is 10 so that each trainee can practice in ideal conditions.
Applicants will benefit from the molecular biology platform of the University Hospital of Nîmes. They will practice under the guidance of experts. In order to maximize and facilitate exchanges, experts will be present with trainees for the whole day.
Deadline for application: Monday April 11th, 2011.
Registration fees : 250,00 €.
Programme of the training school
Detection of mutations in MPN: theory, practice (qPCR, HRM)
TUESDAY MAY 10th, 2011 |
|
| 16:00 – 18:00 Welcome and orientation | |
WEDNESDAY MAY 11th, 2011 |
|
JAK2V617F quantification by real time PCR |
|
| 08:30 Morning session: lectures | |
| Presentation of the COST Training School | Pr LAVABRE-BERTRAND, Nîmes, France |
| Real time PCR quantification : theory | Dr PALLISGAARD, Odensee, Denmark |
| JAK2V617F quantification | Dr LIPPERT, Bordeaux, France |
| Coffee break | |
| Detection & quantification of the JAK2V617F mutation by LNA clamped PCR |
Dr EL HOUSNY, Brussels, Belgium |
| Lunch at the University Hospital restaurant | |
| Afternoon session: practice | |
| JAK2V617F quantification using different techniques | |
THURSDAY MAY 12th, 2011 |
|
JAK2, except V617F, and MPL mutations |
|
| 09:00 Morning session: lectures | |
| Nucleic acid hybridization and melting theory | Dr CLAIR Montpellier, France |
| Coffee break | |
| JAK2 and MPL mutations detection by HRM (High Resolution Melting analysis) |
Dr CARILLO Nîmes, France |
| Lunch at the University Hospital restaurant | |
| Afternoon session: practice | |
| HRM | |
| 18:00 Departure for Gala dinner at the “Pont du Gard” (famous roman aqueduc) | |
FRIDAY MAY 13th, 2011 |
|
Predisposition mutations |
|
| 09:00 Morning session: lectures | |
| Title to be precised | Dr BELLOSILLO Barcelona, Spain |
| Coffee break | |
| Title to be precised | Dr MURATI Marseille, France |
| Adjourn | |
| Lunch possible, on request, at the University Hospital restaurant | |
Place of Meeting
Laboratoire de Cytologie Clinique et Cytogénétique
Hall5, niveau 0
Place du Pr DEBRE
30 029 Nîmes Cedex 9 – France
Tel : 04 66 68 68 68
To visualize the CHU location, click : here.
Contact with the organizers
Before to contact the organizers, please read carefully the information provided on the web site. Questions may ONLY be asked through the contact webpage.
How to register, submit a contribution, pay registration fees, get invoice ?
For registration, submission of contribution … select Registration on the menu bar.
CHU CAREMEAU
Hotels and accommodation
Each participant has to make his/her own room reservation.
Please mention the code “CHU NIMES” to benefit from our special rates.
Click on the Hotel name to get on the Hotel website.
- Hôtel VATEL ****, close to the CHU, 140 rue Vatel – BP 7128 – 30913 Nîmes Cedex 02, with a special rate per night breakfast included, from 89 to 99 € single room, from 105 to 115 € double/twin room. Please mention the code “CHU NIMES” to benefit from this special price. To visualize the hotel location, click : here. For booking, send a mail to reservation.nimes@vatel.fr.
- Hôtel CESAR **, close to the train SNCF station and city center, 17, avenue Feuchères – 30000 Nîmes, with a rate per night from 40 to 50 €, breakfast 7 €. To visualize the hotel location, click : here. For booking, send a mail to nimeshotelcesar@orange.fr.
- Hôtel 1ere Classe, 83 rue de la République – 30900 Nîmes, with a rate per night of 48 €, breakfast 4.80 €. To visualize the hotel location, click : here. For booking, send a mail to nimes@premiereclasse.fr.
Moving down town …
Bus information : ttp://www.tangobus.fr
Lines F and G leads to CHU. Participants will get TANGO tickets for traveling May 11, 12 and 13.
